Abstract

We have constructed a detailed physical map of PB2, a temperate bacteriophage of Agrobacterium tumefaciens. The restriction endonucleases Bam HI (3 sites), Eco RI (22 sites), Hind III (19 sites), Hpa I (5 sites), Xba I (1 site), and Xho I (1 site) were used to elucidate the map of infectious PB2 DNA. The map was generated by reciprocal sequential digestions and analysis of partial digestion products of isolated restriction fragments. We have determined the genome size as 66.35 +/- 1.71 kb. The potential of PB2 as a phage-based shuttle vector for the A. tumefaciens-plant transformation system is discussed.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.