Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera.

Anusha Rai,Gupta Deepshikha,Chintalapati Venkata Ramana,Chintalapati Sasikala,Nandardhane Smita,Uppada Jagadeeshwari

doi:10.3389/fmicb.2021.677842

Abstract

The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Roseomonas. Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing Roseomonas species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (dDDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus Roseomonas and other interspersing members of the family Acetobacteraceae were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus Roseomonas and propose six novel genera as Pararoseomonas gen. nov., Falsiroseomonas gen. nov., Paeniroseomonas gen. nov., Plastoroseomonas gen. nov., Neoroseomonas gen. nov., and Pseudoroseomonas gen. nov.

Highlights

The genus Roseomonas comes under the family Acetobacteraceae, the class Alphaproteobacteria, and the phylum Proteobacteria (Rihs et al, 1993)
The 16S rRNA gene-based phylogenetic tree showed that the genus Roseomonas was polyphyletic and was segregated into seven major clades (Figure 1)
Genus Roseomonas has been widely studied as the members have clinical and environmental significances (Rihs et al, 1993; Han et al, 2003; Romano-Bertrand et al, 2016; Subhash et al, 2016)

Summary

Introduction

The genus Roseomonas comes under the family Acetobacteraceae, the class Alphaproteobacteria, and the phylum Proteobacteria (Rihs et al, 1993). They may have fundamental functions in supporting various ecological and biogeographical processes They are highly ubiquitous and have been widely isolated from clinical specimens like blood, wounds, and genitourinary samples (Bibashi et al, 2000; Han et al, 2003) and environmental samples like contaminated oil sediments (Subhash and Lee, 2018), water (Gallego et al, 2006), soil (Kim and Ka, 2014), and air (Kim et al, 2013). Members of this genus are aerobic, Gram-negative, pink pigmented, and non-fermentative (Rihs et al, 1993). R. fauriae, described by Rihs et al (1998), is a later heterotypic synonym of Azospirillum brasilense (Tarrand et al, 1978; Helsel et al, 2006)

Methods

Results

Conclusion