Phylogeography and population expansion of Western Himalayan highly-erratically migratory painted lady butterfly (Lepidoptera: Nymphalidae: Vanessa cardui)

Abstract

• We examined 91 Vanessa cardui from Western Himalayas (Uttarakhand), India. • We used COI gene sequencing for molecular variation. • We observed late species colonization in the novel area. • V. cardui populations are genetically less differentiated. • V. cardui showed a high level of gene flow after initial colonization. Understanding population genetics, genetic diversity and the migration pattern of ecologically important species is crucial for effective conservation plans. We studied mitochondrial cytochrome c oxidase subunit-I (CO I ) gene variation in painted lady butterfly ( Vanessa cardui ) from different altitudinal gradients of Uttarakhand (Western Himalayas), India. We sequenced CO I ) gene of 91 samples and 22 CO I sequences retrieved from GenBank as representatives of different localities for comparative analysis. We observed a total of 16 haplotypes, of which seven haplotypes were observed in Uttarakhand with intra-species sequence divergence ranges from 0.001 to 0.009. Haplotype (Hd) and nucleotide diversity (π) were comparatively low and averaging 0.635 and 0.002, respectively. The neutrality test, mismatch distribution graph and observed pattern of diversity indices (Hd and π) revealed population expansion. It was further confirmed by the Median Joining Network, which exhibited a star-like topology from the core haplotype, while the Bayesian phylogenetic tree displayed a mixing of haplotypes from different localities to an identical clade. Haplotype organization in the Bayesian tree and Median Joining Network was not clustered as per their topographical affinity, indicating incomplete lineage sorting and late species population development or colonization in the novel area.