Phylogeographical structure of vaironeTelestes muticellus(Teleostei, Cyprinidae) within three European peri‐Mediterranean districts

Abstract

The phylogeographical pattern of vairone (Telestes muticellus) populations was assessed to test the biogeographical distinction of three peri‐Mediterranean ichthyogeographical districts: Padano‐Venetian (PV), Tuscano‐Latium (TL) and southern France (SF), evaluating the role of Alpine and Apennine barriers in shaping distinct evolutionary lineages. A fragment of mitochondrial DNA (mtDNA) cytochromeb(cytb) was sequenced in 153 specimens from 14 north‐western Italian populations, collected in 10 tributaries of Po River and in four rivers on the Tyrrhenian slope of Ligury, and 32 haplotypes were identified. The phylogenetic analyses confirmed the presence of two distinct clades, an ‘Italian clade’ (T. muticellus) and a ‘French clade’ (T. souffia), showing an average genetic distance of 12.9% (± 1.3 SD) and allopatric distribution. The Nested Clade Analysis (NCA) and the Analysis of Molecular Variance (amova) revealed an isolated gene pool in eastern basins on the Tyrrhenian slope of Ligury. The phylogeographical findings suggest: (i) the lack of permeability of Alpine barrier towards the dispersion across Italian and French hydrographical systems; and (ii) partial permeability of Mediterranean Alps and Apennines through river captures crossing lower watersheds.