Abstract

Both nuclear ribosomal ITS and chloroplast trn L-F sequences were acquired from 13 mulberry genotypes belonging to nine species and three varieties, and one paper mulberry. The later belongs to genus B. papyrifera , designed as outgroup, and were analyzed. Within the genus Morus , the sequence diversity of ITS was much higher than that of trn L-F. The results of phylogenetic analyses based on these data (separately or combined) show that the genus Morus is monophyletic group. Strict consensus tree obtained through the Neighbor-joining method can be divided into five major clades in the genus Morus , according to combined sequence data. M. bombycis, M. alba var. venose formed clades A and B, respectively. Clade C comprises of 5 species; M. rotundiloba, M. atropurpurea, M. mongolica, M. australi , and M. mongolica var. diabolica . Clade D comprises of 3 species; M. wittiorum, M. laevigata , and M. alba . Clade E comprises of 2 species; M. multicaulis , and M.alba var. macrophylla . The results from cluster analysis were basically in agreement with the existing morphologic classification. African Journal of Biotechnology Vol. 4 (6), pp. 563-569, 2005

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