Phylogeny of Catenulida and support for Platyhelminthes

Abstract

Molecular studies have shown that Platyhelminthes is polyphyletic, placing Rhabditophora within Lophotrochozoa, whereas Acoela and Nemertodermatida are separate early bilaterian branches. However, there has been little evidence to support the position of Catenulida, a group that was traditionally classified within Platyhelminthes. In Ehlers’ pioneering cladistic system of the Platyhelminthes they were placed as the earliest clade. Other morphologists have considered the Catenulida as an early bilaterian clade separate from Rhabditophora, a position that was supported in an early molecular study. Subsequent molecular phylogenetic studies, which placed Catenulida as the sister group of Rhabditophora with no or low branch support, included 18S rDNA data from only one or two catenulid species. The aims of the present study were (1) to test the putative sister-group relationship of Catenulida and Rhabditophora by improving the taxon sampling of molecular data spanning a larger part of catenulid taxonomic diversity and (2) to provide a phylogenetic framework for the systematization of Catenulida. Twelve catenulid species were sampled around Sweden. Both the 18S rDNA gene and the 28S rDNA gene were sequenced and analysed in a Metazoa-wide data set within parsimony and Bayesian frameworks. The results unambiguously support Catenulida as the sister group of Rhabditophora within Lophotrochozoa. Parsimony-based inferences about the common ancestor of Catenulida and Rhabditophora are presented. A definition of the name Platyhelminthes is suggested.