Phylogeny Analysis of gyrB Gene and 16S rRNA Genes of Pseudomonas aeruginosa Isolated from Iraqi Patients

Abstract

Infection caused by Pseudomonas aeruginosa is one of the major problems in hospitalized patients which are related to the high mortality. The DNA gyrase B, gyrB reading gene sequence method provides a fast and efficient system for bacterial identification and diagnosis, taxonomic analysis and monitoring of bacteria in the natural environment. Evolution analysis was performed using gene nucleotide sequences for gyrB and 16S rRNA genes. PCR amplifiers were used for the genes under study and their genetic sequences were read. The evolutionary tree was drawn based on the genetic sequences of the classification of P. aeruginosa, compared to the analysis of the 16S rRNA genes, gyrB sequences showed a greater evolutionary deviation of bacteria and may be useful for distinguishing between closely related species. Sequence analysis of 16S rRNA is accurate for identifying unknown bacteria to the genus level. However, the variable gyrB sequence analysis can identify unknown bacteria to the species level. Together with the 16S rRNA analysis, gyrB sequence analysis is considered a useful tool to build the evolutionary relationships of bacteria, especially for the classification of converging bacterial species and controlling the invasive Patho Micobial infection treatment in the hospital.