Phylogenomic Characterization of a Novel Corynebacterium Species Associated with Fatal Diphtheritic Stomatitis in Endangered Yellow-Eyed Penguins.

Sarah C Saunderson,Jim Watts,Kate M Mcinnes,David A Wilkinson,Vartul Sangal,Anne C Midwinter,Imen Nouioui,Melanie J Young,Nicola Segata

doi:10.1128/msystems.00320-21

Abstract

ABSTRACTYellow-eyed penguins, Megadyptes antipodes, are an endangered species that are endemic to New Zealand. Outbreaks of diphtheritic stomatitis have caused significant mortality for this species, especially among young chicks. In this study, we isolated 16 Corynebacterium sp. isolates from the oral cavities of 2- to 14-day-old chicks at a range of infection stages and sequenced the genomes to understand their virulence mechanisms. Phylogenomic and matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) characterization indicate that these strains belong to a novel Corynebacterium species. A simple multiplex PCR-based diagnostic assay has been developed to identify these strains rapidly and reliably. Similar to other corynebacteria, genomic islands and prophages introduced significant diversity among these strains that has potentially led to minor functional variations between the two lineages. Despite the presence of multiple corynebacterial virulence genes and a spaDEF-type pilus gene cluster among these strains, the survival rate was much higher in Galleria mellonella larvae than in those inoculated with Corynebacterium ulcerans NZRM 818 and Corynebacterium pseudotuberculosis NZRM 3004. Therefore, these strains are opportunistic pathogens causing high mortality among young penguin chicks due to a less-developed immune system.IMPORTANCE Yellow-eyed penguins, Megadyptes antipodes, are endangered species with a sharp decline in the numbers of breeding pairs over the last 2 decades. Diphtheritic stomatitis, characterized by a thick fibrinopurulent exudate in the oral cavities and symptoms, including inanition and significant weight loss, is responsible for significant mortality among the young chicks. These chicks are treated with antibiotics, amoxicillin-clavulanic acid or enrofloxacin, but do not always recover from the infection. The pathogen causing these infections and the mechanism of pathogenesis are unclear. This study has identified a novel Corynebacterium species to be associated with diphtheritic stomatitis in yellow-eyed penguins with potential virulence genes that are likely involved in pathogenesis. Importantly, a gene encoding an exotoxin, phospholipase D, is present among these strains. The inactivated form of this enzyme could potentially be used as an effective vaccine to protect these penguins from infection.

Highlights

Yellow-eyed penguins, Megadyptes antipodes, are an endangered species that are endemic to New Zealand
We report the findings on novel Corynebacterium strains isolated from lesions derived from the oral cavities of 2- to 14day-old yellow-eyed penguin chicks
Yellow-eyed penguin chicks (2 to 14 days old) at a range of diphtheritic stomatitis infection stages were identified within breeding regions of the Otago Peninsula (Table 1; Fig. 1; Fig. S1)

Summary

Introduction

Yellow-eyed penguins, Megadyptes antipodes, are an endangered species that are endemic to New Zealand. Diphtheritic stomatitis, characterized by a thick fibrinopurulent exudate in the oral cavities and symptoms, including inanition and significant weight loss, is responsible for significant mortality among the young chicks These chicks are treated with antibiotics, amoxicillin-clavulanic acid or enrofloxacin, but do not always recover from the infection. This study has identified a novel Corynebacterium species to be associated with diphtheritic stomatitis in yellow-eyed penguins with potential virulence genes that are likely involved in pathogenesis. Diphtheritic stomatitis is responsible for high mortality rates among 1- to 28-day-old chicks, which is characterized by a thick cream-colored fibrinopurulent exudate in the oral cavities and symptoms, including inanition and significant weight loss leading to death [7, 8]. We report the findings on novel Corynebacterium strains isolated from lesions derived from the oral cavities of 2- to 14day-old yellow-eyed penguin chicks. The phylogenomic characterization identified two distinct lineages with potential virulence genes, which may be associated with diphtheritic stomatitis

Methods

Results

Conclusion