Phylogenomic Analysis Reveals That Arthrobacter mysorens Nand and Rao 1972 (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016 are Later Heterotypic Synonyms of Arthrobacter nicotianae Giovannozzi-Sermanni 1959 (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016.

Abstract

We described the comparative genomic analysis to delineate the taxonomic relationship between the two Glutamicibacter species Glutamicibacter mysorens NBRC 103060T and Glutamicibacter nicotianae NBRC 14234T. Phylogenetic tree based on concatenated ribosomal protein marker genes showed both species clade together. The average nucleotide identity (ANI) values between G. mysorens NBRC 103060T and G. nicotianae NBRC 14234T ranged from 97.23 to 97.97%. Further, the average amino acid identity (AAI) between two strains were more than 97.61%. The ANI, AAI and in silico DNA-DNA hybridization (isDDH) data were higher than the threshold value for bacterial species delineation. The two strains have identical profile of fatty acids, sugars and lipid composition; and overall similar phenotypic characteristics. It therefore becomes evident that the two species actually belong to the same species. Arthrobacter nicotianae (Giovannozzi-Sermanni 1959) (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016 have priority over the names Arthrobacter mysorens (Nand and Rao 1972) (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016, therefore we proposed that Glutamicibacter mysorens Busse 2016 is a later heterotypic synonym of Glutamicibacter nicotianae Busse 2016. The type strain is ATCC 15236T = CCM 1648T = BCRC (formerly CCRC) 11219T = CCUG 23842T = CDA 883T = CIP 82.107T = DSM 20123T = HAMBI 1859T = IAM 12342T = IFO (now NBRC) 14234T = IMET 10353T = JCM 1333T = LMG 16305T = NCIMB 9458T = NRIC 0153T.