Abstract
NADH:quinone-oxidoreductase of type I (EC 1.6.5.2, Complex I, hereafter NDH-I) is the largest enzyme in the respiratory chain of mitochondria. It couples the transfer of two electrons from NADH to the membrane ubiquinone pool with the translocation of (supposedly) four protons across the membrane [1-3]. The genes of NDH-1 are found in many bacterial and archaeal genomes [4], although the products of these genes were functionally characterized only in a few cases.
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