Abstract
Bats, order Chiroptera, are one of the largest monophyletic clades in mammals. Based on morphology and behaviour bats were once differentiated into two suborders Megachiroptera and Microchiroptera Recently, researchers proposed alternative views of chiropteran classification (suborders Yinpterochiroptera and Yangochiroptera) based on morphological, molecular and fossil evidence. Since genome-scale data can significantly increase the number of informative characters for analysis, transcriptome RNA-seq data for 12 bat taxa were generated in an attempt to resolve bat subordinal relationships at the genome level. Phylogenetic reconstructions were conducted using up to 1470 orthologous genes and 634,288 aligned sites. We found strong support for the Yinpterochiroptera-Yangochiroptera classification. Next, we built expression distance matrices for each species and reconstructed gene expression trees. The tree is highly consistent with sequence-based phylogeny. We also examined the influence of taxa sampling on the performance of phylogenetic methods, and found that the topology is robust to sampling. Relaxed molecular clock estimates the divergence between Yinpterochiroptera and Yangochiroptera around 63 million years ago. The most recent common ancestor of Yinpterochiroptera, corresponding to the split between Rhinolophoidea and Pteropodidae (Old World Fruit bats), is estimated to have occurred 60 million years ago. Our work provided a valuable resource to further explore the evolutionary relationship within bats.
Highlights
IntroductionOrder Chiroptera, are one of the largest monophyletic clades in mammals. Based on morphology and behaviour bats were once differentiated into two suborders Megachiroptera and Microchiroptera Recently, researchers proposed alternative views of chiropteran classification (suborders Yinpterochiroptera and Yangochiroptera) based on morphological, molecular and fossil evidence
Bats, order Chiroptera, are one of the largest monophyletic clades in mammals
A reconsideration of morphological, behavioural and molecular evidence demonstrates that there are two suborders of bats, Yinpterochiroptera and Yangochiropterathat do not coincide with the previous subordinal classificaiton[5,6]
Summary
Order Chiroptera, are one of the largest monophyletic clades in mammals. Based on morphology and behaviour bats were once differentiated into two suborders Megachiroptera and Microchiroptera Recently, researchers proposed alternative views of chiropteran classification (suborders Yinpterochiroptera and Yangochiroptera) based on morphological, molecular and fossil evidence. Bats belong to the order Chiroptera, one of the largest monophyletic clades in mammals They constitute ~20% of living mammalian species, arranged in 20 families[1]. Phylogenomic analysis based on genome sequencing data support the classification of living bats in Yinpterochiroptera and Yangochiroptera[7]. O’Leary et al claimed that living echolocating bats were monophyletic[8] They based this on morphological data set and published molecular sequence data the bat genome data set is rich in the number of loci, it is not comprehensive in taxon sampling, an important component for accurately estimating phylogeny[9]. After evaluating the influence of taxa sampling on the performance of phylogenetic methods, we found strong support for the Yinpterochiroptera-Yangochiroptera classification. Species Cynopterus sphinx Rousettus leschenaultii Aselliscus stoliczkanus Hipposideros pratti Rhinolophus pusillus Megaderma lyra Rhinopoma hardwickei Taphozous melanopogon Tadarida teniotis Murina leucogaster Myotis ricketti Scotophilus kuhlii
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