Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella pneumonia and Enterobacter aerogenes Isolated from patients with Ocular eye infection in Wasit province, Iraq

Abstract

In Iraq and other developing countries, ocular infections are a significant public health concern, particularly gram-negative bacterial infections, which are common. The emergence of multidrug resistance is a critical concern because it can lead to treatment failure when bacterial strains become resistant to antimicrobial drugs. This study aimed to investigate, Phylogenetic tree analysis of Klebsiella pneumonia and Enterobacter aerogenes isolated from patients with Ocular eye infection. The tree provided evidence of a unique species that was represented by a single nucleic acid sequence. This species encompassed both Klebsiella pneumoniae and Enterobacter aerogenes. Through phylogenetic tree analysis, it was determined that the 16SrRNA gene exhibited effective discriminatory power in identifying these bacterial strains. Based on the sequencing data, it can be inferred that there is a significant sequence similarity between the Klebsiella pneumoniae samples and strains from Bangladesh. Similarly, Enterobacter aerogenes showed high sequence similarity with strains from India and Iraq, indicating a shared species affiliation.