Abstract
Dunaliella strains were isolated from a key site for salt pro duction in Vietnam (Vinh Hao, Binh Thuan province). The strains were identified based on Int ernal Transcribed Spacer (ITS) markers. The phylogenetic tree revealed these strains belong to the clades of Dunaliella salina and Dunaliella viridis . Results of this study confirm the ubiquitous nature of Dunaliella and suggest that strains of Dunaliella salina might be acquired locally worldwide for the produc tion of beta-carotene. The identification of these species infers the presence of other Dunaliella species ( Dunaliella tertiolecta , Dunaliella primolecta , Dunaliella parva ), but further investigation would be required to c onfirm their presence in Vietnam. We anticipate the physiological and biochemical charac teristics of these local species will be compared w ith imported strains in a future effort. This will faci litate selection of strains with the best potential for exploitation in the food, aquaculture and biofuel i ndustries. The Dunaliella strains isolated and identified in this study are maintained at the Laboratory of Alga l Biotechnology, International University and will be made available for research and educational institu tions.
Highlights
Cells of Dunaliella are generally ovoid, 4-15 μm wide and 6-25 μm long, but depending on stages of Unicellular green algae Dunaliella belong to the Chlorophytes (Oren, 2005)
Various isolates grown under different salinities were scanned to match the morphological descriptions of Dunaliella (Teodoresco, 1906; Butcher, 1959; Massjuk, 1973a; 1973b; Polle et al, 2009)
We identified Dunaliella salina and Dunaliella viridis for in Vietnam based on morphology, physiology and molecular markers
Summary
Cells of Dunaliella are generally ovoid, 4-15 μm wide and 6-25 μm long, but depending on stages of Unicellular green algae Dunaliella belong to the Chlorophytes (Oren, 2005). The algae was first described (Dunal, 1838), but it was not until 1905 that the name Dunaliella was given by Teodoresco (1905). There are currently 23 recognized species of Dunaliella growth or development and environmental conditions, the cell shape can vary from ovoid, ellipsoidal, cylindrical, pyriform, or fusiform to almost spherical (Butcher, 1959; Massjuk, 1973a; 1973b). Dunaliella cells are motile with two long flagella.
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