Abstract

In crosses between T. tauschii (Dt) accesions, their polymorphic gliadin forms were inherited as blocks of gliadin components -Gli-Dt1, Gli-Dt2 — as single Mendelian characters. From the progeny of four tri-parental crosses (test-crosses), HMW glutenin subunits derived from T. tauschii (Glu-Dt1) segregated as alleles of the Glu-D1 locus in bread wheat. In three of the tri-parental crosses, a small proportion (2.5%) of the progeny with atypical segregation patterns, were identified through somatic chromosome counts, to be aneuploids (1.9% hypoploids and 0.6% hyperploids). Chromosomal mapping studies revealed that the synteny of genes for HMW glutenin subunits and gliadins in T. tauschii are conserved in the D genome ‘homologue’ (chromosome 1D) of T. aestivum. The map distance between the Glu-D1/-Dt1 and Gli-D1/-Dt1 loci was calculated to be 63.5 cM, while a linkage to the centromere of 7.7–9.7 cM was estimated for the Glu-D1/-Dt1 locus.

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