Abstract
Macaca fascicularis is one of the most common primates found in Malaysia; however, they are under-appreciated and under-studied, especially in the taxonomic field. The subspecies M. fascicularis laeta was previously hypothesized to be distributed in Pulau Tioman and Pulau Tinggi. Thus, in this study, we aim to verify the phylogenetic position of long-tailed macaque in Pulau Tinggi through a molecular approach based on the D-loop region of mitochondrial DNA using Neighbour-Joining (NJ), Maximum Parsimony (MP) and Bayesian Inference. Three samples were successfully analyzed with a 99% bootstrap value in NJ, 99% bootstrap value in MP and 1.00 posterior probability in Bayesian Inference for Pulau Tinggi’s clade. The phylogenetic analysis concluded that Pulau Tinggi’s population is similar to the mainland population, which is the subspecies M. f. fascicularis, with an estimated time of divergence at ~0.299 million years ago. Overall, this study generally provides baseline data to aid in the future translocation process and conservation management of M. fascicularis in Pulau Tinggi and Malaysia.
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