Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistant Escherichia coli originated from the broiler meat supply chain in Bogor, Indonesia.

Irma Rahayuningtyas,Maria Fatima Palupi,Agustin Indrawati,Istiyaningsih Istiyaningsih,I Wayan Teguh Wibawan

doi:10.14202/vetworld.2020.1807-1814

Irma Rahayuningtyas, Maria Fatima Palupi + Show 3 more

Open Access

https://doi.org/10.14202/vetworld.2020.1807-1814

Copy DOI

Abstract

Background and Aim:Pathogenic Escherichia coli contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic E. coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) showed a close genetic relationship and may share virulence genes. This study aimed to determine the phylogenetic group and virulence gene profiles in colistin-resistant E. coli obtained from the broiler meat supply chain in Bogor, West Java, Indonesia.Materials and Methods:Fifty-eight archive isolates originated from the cloacal swab, litter, drinking water, inside plucker swab, fresh meat at small scale poultry slaughterhouses, and traditional markets were used in this study. All the isolates were characterized by a polymerase chain reaction to determine the phylogenetic group (A, B1, B2, or D) and virulence gene profiles with APEC marker genes (iutA, hlyF, iss, iroN, and ompT).Results:Phylogenetic grouping revealed that the isolates belong to A group (34.48%), D group (34.48%), B1 group (17.24%), and B2 group (13.79%). The virulence gene prevalence was as follows: iutA (36%), hlyF (21%), ompT (21%), iroN (10%), and iss (9%). The B2 group presented with more virulence genes combinations. iroN, hlyF, and ompT genes were positively associated with the B2 group (p≤0.05).Conclusion:Our results highlight the role of colistin-resistant E. coli originated from the broiler meat supply chain as a potential reservoir for human ExPEC virulence genes.

Highlights

Most Escherichia coli are commensal bacteria, but some strains are pathogenic in humans and warmblooded animals
Our results highlight the role of colistin-resistant E. coli originated from the broiler meat supply chain as a potential reservoir for human extraintestinal pathogenic E. coli (ExPEC) virulence genes
This study provides the results of the determination of phylogenetic groups and virulence gene profiles of colistin-resistant E. coli originated from the broiler meat supply chain in Bogor, West Java, Indonesia

Summary

Introduction

Most Escherichia coli are commensal bacteria, but some strains are pathogenic in humans and warmblooded animals. One of the critical pathogenic E. coli is extraintestinal pathogenic E. coli (ExPEC) This pathotype may cause systemic infections in humans and animals. ExPEC subpathotypes include uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), sepsis-associated E. coli (SePEC), and avian pathogenic E. coli (APEC). UPEC causes urinary tract infections (UTI), NMEC leading to neonatal meningitis, and SePEC, which causes sepsis in humans [1]. Pathogenic Escherichia coli contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic E. coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) showed a close genetic relationship and may share virulence genes. This study aimed to determine the phylogenetic group and virulence gene profiles in colistin-resistant E. coli obtained from the broiler meat supply chain in Bogor, West Java, Indonesia

Objectives

Methods

Results

Discussion

Conclusion