Phylogenetic comparison of porcine circovirus type 2 (PCV2) and porcine reproductive respiratory syndrome virus (PRRSV) strains detected in domestic pigs until 2008 and in 2012 in Croatia.

Jelena Prpić,Tomislav Bedeković,Besi Roić,Tomislav Keros,Željko Cvetnić,Lorena Jemeršić,Dragan Brnić

doi:10.1186/2046-0481-67-9

Jelena Prpić, Tomislav Bedeković + Show 5 more

Open Access

https://doi.org/10.1186/2046-0481-67-9

Copy DOI

Journal: Irish veterinary journal	Publication Date: May 14, 2014
Citations: 22	License type: cc-by

Affiliation: Croatian Veterinary Institute

Abstract

BackgroundPorcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) have been present for the last 2 decades in Croatia, causing large economical losses in the pig production. The clinical features of the infections are mostly manifested by the development of respiratory problems, weight loss and poor growth performance, as well as reproductive failure in pregnant sows. Even though the infections are continuously recognized in some regions in Croatia, the heterogeneity of the detected viral strains from 2012 has not yet been investigated. The objective of this study was to compare virus strains of PCV2 and PRRSV detected until 2008 in Croatia with strains isolated in 2012 to gain a better epidemiological understanding of these two infections.ResultsPCV2 and PRRSV strains detected in 2012 in fattening pigs from regions where these two diseases have been previously described were compared to strains that have been detected in the same regions within the past two decades. The phylogenetic analysis revealed that the circulating PCV2 and PRRSV strains are distantly related to the previously described Croatian viral strains. However, when compared to known isolates from the GenBank a high genetic identity of PRRSV isolates with isolates from Hungary, Denmark and the Netherlands was found.ConclusionThe results of this study reveal that even though PCV2 and PRRSV are constantly present in the investigated regions in Croatia, the viral strains found in 2012 genetically differ from those detected in earlier years. This indicates that new entries into the pig population appeared with regard to both infections, probably as a result of pig trade.

Highlights

Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) have been present for the last 2 decades in Croatia, causing large economical losses in the pig production
The amplification of PCV2 genome regions from samples originating from the two farms with clinical signs of PCV2 (CRO-PCV2-1 to CRO-PCV2-10) resulted in clear PCR products with the expected molecular size
RT-PCR assay, using a primer pair specific for a sequence within the PRSSV ORF7, resulted in strong amplification signals with the expected molecular size in all pooled samples collected from swine with clinical signs of PRRS (CROPRRSV-1 to CRO-PRRSV-11)

Summary

Introduction

Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) have been present for the last 2 decades in Croatia, causing large economical losses in the pig production. The objective of this study was to compare virus strains of PCV2 and PRRSV detected until 2008 in Croatia with strains isolated in 2012 to gain a better epidemiological understanding of these two infections. Two types of PCV have been described, namely PCV1 and PCV2. Their DNA sequence homology is 68 to 76% [5]. Boisseson et al [8] found that the variations among the PCV2 genomic sequences are mainly due to the variability within ORF2

Objectives

Methods

Results

Conclusion