Abstract
The genus Dactylis, an important forage crop, has a wide geographical distribution in temperate regions. While this genus is thought to include a single species, Dactylis glomerata, this species encompasses many subspecies whose relationships have not been fully characterized. In this study, the genetic diversity and phylogenetic relationships of nine representative Dactylis subspecies were examined using SSR and IT-ISJ markers. In total, 21 pairs of SSR primers and 15 pairs of IT-ISJ primers were used to amplify 295 polymorphic bands with polymorphic rates of 100%. The average polymorphic information contents (PICs) of SSR and IT-ISJ markers were 0.909 and 0.780, respectively. The combined data of the two markers indicated a high level of genetic diversity among the nine D. glomerata subspecies, with a Nei’s gene diversity index value of 0.283 and Shannon’s diversity of 0.448. Preliminarily phylogenetic analysis results revealed that the 20 accessions could be divided into three groups (A, B, C). Furthermore, they could be divided into five clusters, which is similar to the structure analysis with K = 5. Phylogenetic placement in these three groups may be related to the distribution ranges and the climate types of the subspecies in each group. Group A contained eight accessions of four subspecies, originating from the west Mediterranean, while Group B contained seven accessions of three subspecies, originating from the east Mediterranean.
Highlights
The genus Dactylis L. comprises wind-pollinated and out-crossing cool-season perennial grasses belonging to the grass tribe Poeae, the subfamily Pooideae, and the family Poaceae [1,2]
Due to the high polymorphism rates and efficiency of the Simple sequence repeat (SSR) marker system, it has been widely used for genetic mapping [25,41,42], phylogenetic analysis [32,43], and evaluating genetic diversity [44,45]
According to Vaiman et al [46], loci polymorphisms can be divided into three levels based on their information contents: high (PIC > 0.5), medium (0.5 > polymorphic information contents (PICs) > 0.25), and low (PIC < 0.25)
Summary
The genus Dactylis L. comprises wind-pollinated and out-crossing cool-season perennial grasses belonging to the grass tribe Poeae, the subfamily Pooideae, and the family Poaceae [1,2]. Dactylis is native to the northern hemisphere and can be found throughout Europe, temperate and tropical Asia, North Africa, and the Canary Islands [6,7,8]. It has been an important forage grass for more than 100 years in almost all temperate regions of the world. In many localities the two types are sympatric [11], hexaploids exist across limited ranges in Libya and western Egypt [11,12,13,14]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
