Phylogenetic Analysis of Uromyces Species Infecting Grain and Forage Legumes by Sequence analysis of Nuclear Ribosomal Internal Transcribed Spacer Region

Abstract

DNA sequence analysis of the nuclear ribosomal internal transcribed spacer region (ITS) was performed to determine phylogenetic relationship between 49 isolates of rusts infecting grain and forage legumes. Isolates were collected from different hosts and distinct geographic origins and represent eight species of Uromyces: U. anthyllidis, U. appendiculatus, U. ciceris-arietini, U. minor, U. pisi, U. striatus, U. viciae-fabae and U. vignae. ITS sequences revealed length polymorphisms and variation in DNA sequence that were used to characterize phylogenetic relationships by maximum parsimony, maximum likelihood and Bayesian analyses which in general agreed revealing the presence of four clearly distinct clades. Clade one included the isolates causing rust on chickpea, fenugreek and alfalfa. Clade two was composed by rust isolates of field clover and pea plants, while the third clade was formed by bean and cowpea isolates. Clade four was the largest and included all the rust isolates infecting faba bean. Within this clade, the highly supported subclusters of U. viciae-fabae collected on Lens culinaris, U. viciae-fabae collected on Vicia sativa and U. viciae-fabae collected on Lathyrus palustris suggest an ongoing process of host specialization.