Phylogenetic analysis of the ATP-binding cassette proteins suggests a new ABC protein subfamily J in Aedes aegypti (Diptera: Culicidae)

Janaina Figueira-Mansur,Ana Claudia A. Melo,Carlos G. Schrago,Evelyn S. L. Alvarenga,Tiago S. Salles,Monica F. Moreira,Brenda M. Vasconcellos

doi:10.1186/s12864-020-06873-8

Abstract

BackgroundWe performed an in-depth analysis of the ABC gene family in Aedes aegypti (Diptera: Culicidae), which is an important vector species of arthropod-borne viral infections such as chikungunya, dengue, and Zika. Despite its importance, previous studies of the Arthropod ABC family have not focused on this species. Reports of insecticide resistance among pests and vectors indicate that some of these ATP-dependent efflux pumps are involved in compound traffic and multidrug resistance phenotypes.ResultsWe identified 53 classic complete ABC proteins annotated in the A. aegypti genome. A phylogenetic analysis of Aedes aegypti ABC proteins was carried out to assign the novel proteins to the ABC subfamilies. We also determined 9 full-length sequences of DNA repair (MutS, RAD50) and structural maintenance of chromosome (SMC) proteins that contain the ABC signature.ConclusionsAfter inclusion of the putative ABC proteins into the evolutionary tree of the gene family, we classified A. aegypti ABC proteins into the established subfamilies (A to H), but the phylogenetic positioning of MutS, RAD50 and SMC proteins among ABC subfamilies—as well as the highly supported grouping of RAD50 and SMC—prompted us to name a new J subfamily of A. aegypti ABC proteins.

Highlights

We performed an in-depth analysis of the ATP-binding cassette (ABC) gene family in Aedes aegypti (Diptera: Culicidae), which is an important vector species of arthropod-borne viral infections such as chikungunya, dengue, and Zika
The BLASTp search on the A. aegypti genome retrieved 62 complete proteins that were identified as ABC transporters when submitted to the National Center for Biotechnology Information (NCBI) Conserved Domain Database
The single exception was the clade with subfamilies ABCA and ABCH that were grouped with maximum statistical support

Summary

Introduction

We performed an in-depth analysis of the ABC gene family in Aedes aegypti (Diptera: Culicidae), which is an important vector species of arthropod-borne viral infections such as chikungunya, dengue, and Zika. The ATP-binding cassette (ABC) transporters constitute a diverse gene family consisting of proteins found in all cellular organisms and participating in several different biological pathways [1] Among these processes, the ABC transporters are mostly involved in extra and intracellular trans membrane ATP energy driven traffic of molecules such as lipids, amino acids, hormones and xenobiotics [2, 3]. Besides presenting eukaryotic ABC subfamilies A to G, exhibit a heterogeneous and extensive group of ABC proteins that bear similarities to the components of prokaryotic multisubunit ABC transporters This group was named subfamily I and includes NBD and TMD domains and homologues of soluble cytosolic proteins that interact with NBDs as well as putative substrate-binding proteins similar to the periplasmic binding proteins [10]

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Conclusion