Abstract
Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. Determined sequences from 18 specimens were registered into the GenBank and accession numbers were obtained. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances and tree construction with 1000 bootstrapped replications were conducted using MEGA 6. Maximum likelihood trees were constructed by the HKY model. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. Mongolian sequences from the same and adjacent locations were clustered together.
 European sequences were clustered together, additionally two sequences from south western China and two sequences from south eastern China were also clustered. Additionally, 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population.
Highlights
Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags were subjected to DNA sequencing
Among types of DNA, mitochondrial DNA has been used extensively as it has a high copy of mitochondria in cell
18 complete sequences of Mongolian wild boar mitochondrial 12S rRNA gene from 10 locations were compared to 35 complete sequences of mitochondrial 12S rRNA gene of wild boars available at NCBI GenBank
Summary
Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population. Numerous molecular techniques have been developed based on the use of mitochondrial 12S rRNA gene, such as DNA hybridization [1], restriction fragment length polymorphism [6], real time PCR [5] and so forth
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