Phylogenetic analysis of Endophytes from Bitter Melon (&amp;lt;i&amp;gt;Momordica charantia&amp;lt;/i&amp;gt;) in Guangdong Province

Zide Jiang,Jianghua Huang

doi:10.4236/as.2015.66060

Abstract

This study aims to isolate and identify taxonomic characterization of endophytic fungi from bitter melon in Guangdong province, China. A total of 1172 endophytic fungi are isolated from roots, stems, leaves, flowers, and fruits of healthy plants, and they are classified to 49 taxa based on morphological and molecular features. The results show that endophytic fungi from bitter melon plants exhibit high biodiversity. Arthrinium aureum, A. marii, A. sphaerospermum, Corynascus verrucosus, Curvularia borreriae and C. protuberate have not been recorded in any plants in China. Basidiomycetous endophytes, such as Ceratobasidium sp., and C. cornigerum, are reported in this study for the first time in bitter melon. According to the results of phylogenetic analysis, the rDNA ITS (Internal Transcribed Spacer) sequences can successfully separate species, such as F. solani, F. kyushuense, C. verrucosus, C. globosum, E. rostratum, C. brachyspora, C. verruculosus, C. affinis, P. bougainvilleicola, P. longicolla, P. glabrae, P. verruculosum, P. oxalicum, P. citrinum, P. chermesinum, P. glomerata, A. fumigatus and A. japonicas. Some isolates belonging to A. alternata, C. gloeosporioides, C. cladosporioides, C. brasiliense, C. convolutum, F. proliferatum, F. oxysporum, F. verticillioides, F. equiseti, F. camptoceras and Xylaria, however, require the analysis of others molecular markers to provide better taxonomic resolution. Molecular analyses of rDNA ITS sequences are useful for identification and classification of endophytes of bitter melon, but it is desirable to consider an integrated approach, such as molecular phylogeny, host, colony growth rate, symptoms, and shape of conidia.

Highlights

Endophytes inter- and/or intra-cellularly colonize healthy living tissues of many host plants, typically without causing any noticeable symptoms of disease [1]-[5]
Endophytic fungi from plants have recently been widely accepted as an important source of drugs [6], and they are believed to be rich in diversity and to provide an excellent potential source of biologically active novel compounds [7] [8]
Based on morphological and molecular features, a total of 1172 endophytes were separated from bitter melon, and they were subsequently classified to 49 taxa (Table 1)

Summary

Introduction

Endophytes inter- and/or intra-cellularly colonize healthy living tissues of many host plants, typically without causing any noticeable symptoms of disease [1]-[5]. Endophytic fungi from plants have recently been widely accepted as an important source of drugs [6], and they are believed to be rich in diversity and to provide an excellent potential source of biologically active novel compounds [7] [8]. More than 8600 biologically active compounds have been reported from fungi with various usages [9]. Bitter melon (Momordica charantia), a medicinal food plant, is known to contain charantin (a steroidal glycoside), vicine (a glycoalkaloid), and polypeptide “p” (a 166 residue insulinomimetic peptide) [10]. Extracts of bitter melon have been found to possess novel bioactive natural products like antibacterial, antifungal, anticancer, antioxidant, antivirus and antidiabetes [11]. The medicinal characteristics of bitter melon may result from the capacity of its endophytes producing biologically active secondary metabolites

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Conclusion