Phylogenetic analysis of Anguilla marmorata population in Thua Thien Hue, Vietnam based on the cytochrome C oxidase I (COI) gene fragments

Kieu Thi Huyen,Nguyen Quang Linh

doi:10.1186/s13568-020-01059-7

Kieu Thi Huyen, Nguyen Quang Linh

Open Access

https://doi.org/10.1186/s13568-020-01059-7

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Abstract

The giant mottled eel is a species with high commercial value so overfishing, river management, and water pollution have negatively affected its movement and population numbers. Anguilla marmorata (eel) was listed in the Vietnam Red Data Book 2007 with a description of Vulnerability. This study used a barcode technique to analyze molecular characteristics and build genetic plants based on the cytochrome c oxidase I gene segment isolated from the mitochondrial genome of 48 individuals of A. marmorata collected in five different ecological regions of Thua Thien Hue, Vietnam. The isolated the cytochrome c oxidase I sequence has a length of 843 nucleotides, four base nucleotides of 30.03% Thymine, 25.15% Cytosine, 27.49% Adenine, and 17.43% Guanine. The percentage of Guanine + Cytosine content (42.58%) is acceptable, lower than the Adenine + Thymine content. The replacement capacity of Adenine and Guanine is 22.45% highest, the ratio between Thymine and Guanine; Cytosine and Guanine are the lowest at 2.72%. The establishment of genetically modified plants has shown the high genetic similarity of individuals in eel populations in Thua Thien Hue. The population of Anguilla marmorata eels in Thua Thien Hue, Vietnam is divided into two separate groups that are guided by the migration process and specific ecological. This is particularly important in building strategies to conserve and develop the gene for eel in Vietnam and Thua Thien Hue.

Highlights

Freshwater eels consist of 16 species, included Anguilla marmorata, three of which are further divided into two subspecies (Watanabe 2003; Watanabe et al 2004, 2005)
The BLAST— Basic Local Alignment Search Tool conducted on NCBI and was used to verify and compare with the sequences of A. marmorata, showed that the nucleotide sequences having coverage rates of 100% and 99.76% respectively
There were no difference in the number of C (Cytosine) nucleotides between Anguilla marmorata in Thua Thien Hue compared with two control samples from GenBank (212 correspondings to 25.15%)

Summary

Introduction

Freshwater eels consist of 16 species, included Anguilla marmorata, three of which are further divided into two subspecies (Watanabe 2003; Watanabe et al 2004, 2005). The distribution of freshwater eels are normally in temperate, tropical, and subtropical areas; which is considered to be prevalent nearly worldwide, except for the land masse adjacent to the South Atlantic and the eastern Pacific Oceans. Huyen and Linh A MB Expr (2020) 10:122 distributed in Thua Thien Hue, Vietnam and (2) to assess the diversity and building of genetically generated tree for the population of eel populations in the coastal region of Thua Thien Hue for conservation and control of fishing capture. To detect the genetic variability in A. marmorata, polymerase chain reaction (PCR) amplification applied and sequencing for the partial 5′ hypervariable region of the cytochrome c oxidase subunit 1 (COI) was carried out as a major and powerful barcoding tool widely applied in animals’ population genetics, phylogenies, and taxonomy (Hajibabaei et al 2007)

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