Abstract
During July to August 2013 hop (Humulus lupulus) buds growing in Tasmania (n = 280) and Victoria (n = 200) from nine varieties were sampled and tested for the presence of American hop latent virus (AHLV) by enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction using coat protein gene specific primers. Immunological results showed 153/480 bud samples were positive. RT-PCR resulted in an additional 19 positive samples. Coat protein gene sequence analysis showed 98–99 % identity with AHLV isolates from the USA.
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