Phylogenetic Analysis of a Privately-owned Korean Native Chicken Population Using mtDNA D-loop Variations.

M R Hoque,N R Choi,C Jo,J H Lee,H Sultana,S K Hong,B S Kang,K N Heo

doi:10.5713/ajas.2012.12459

Abstract

The use of Korean native chicken is increasing, and the discovery of new genetic resources is very important from both economic and genetic conservation points of view. In this study, mtDNA D-loop sequences from 272 privately-owned Korean native chickens from a Hyunin farm were investigated. Seventeen nucleotide substitutions were identified from the sequence analysis and they were classified as 6 haplotypes. Previously investigated haplotypes in five Korean native chicken populations have been compared with the Hyunin chicken population. The results indicated that two haplotypes, H10 and H15, in the Hyunin chicken population were not previously identified in other Korean native chicken populations, representing 33.09% (90/272) and 1.1% (3/272) of the Hyunin population, respectively. On the other hand, four other haplotypes were identical to those of a previous study of Korean native chicken populations. This result is indicative of conservation strategies of Hyunin chicken populations for expanding the genetic diversity in the Korean native chicken population.

Highlights

Increased research and genetic profiling of native species is an important component of modern perspectives of genetic diversity conservation
Five lines of Korean native chicken populations were developed in the 1990’s, and this breeding was mainly controlled by a national institution called National Institute of Animal Science (NIAS) in Korea
MtDNA has the advantages of the absence of recombination and rapid nucleotide substitutions compared with genomic DNA sequences (Aquadro and Greenberg, 1983; Lansman et al, 1983; Cann et al, 1984)

Summary

INTRODUCTION

Increased research and genetic profiling of native species is an important component of modern perspectives of genetic diversity conservation. The chicken mtDNA D-loop region was used for the demarcation of haplotypes in the privatelyowned Hyunin native chicken population, and was compared with those of other Korean native chicken breeds, which were previously developed in NIAS. This Hyunin population has distinct feather colors and they were classified as eleven colors. Some of these (red, yellow, black, gray and white) are the same colors identified in Korean native chickens at NIAS. Sci. 26:157-162 basis for an appropriate conservation breeding program for the Korean native chicken

MATERIALS AND METHODS

RESULTS AND DISCUSSION

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