Phylogenetic Analysis and Rapid Detection of Genus Phellinus using the Nucleotide Sequences of 18S Ribosomal RNA

Abstract

Analysis of phylogenetic relationship was performed among Phellinus species based on 18S ribosomal subunit sequence data. Twenty-five strains of 19 Phellinus species including P. linteus were examined in this study. Regions of 18S ribosomal subunit were very conserved, but some variable regions between Phellinus species were observed. The species-specific detection primers, modified by 2 or 3 nucleotides in sense primer were designed based on 18S ribosomal DNA (rDNA) sequence data. The 210 bp PCR bands were detected with annealing temperature 48°C. The 18S 2 F-18S 4 R detection primer set distinguished P linteus from various Phellinus species but some species like P baumii, P weirianius, P rhabarberinus and P pomaceus also had weak reactivity on this primer set. The 18S 3 F-18S 4 R primer set distinguished only P. linteus from various Phellinus species, although sensitivity with this primer set was lower than that of 18S 2 F-18 4 R primer set. These primer sets would be useful for the detection of only P. linteus among unknown Phellinus species rapidly.