Abstract
An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.
Highlights
Contagious ecthyma is a common epitheliotrophic viral disease of sheep, goats, and wild ruminants and is characterized by the formation of papules, nodules, or vesicles that progress into thick crusts or heavy scabs on the lips, gingiva, and tongue
Orf virus infection in dairy goats was identified by clinical diagnosis and PCR
PFhigyulorgeen2etic analysis of different parapoxviruses based on nucleotide sequences of B2L gene (A) and virus interferon resistance (VIR) gene (B) Phylogenetic analysis of different parapoxviruses based on nucleotide sequences of B2L gene (A) and VIR gene (B)
Summary
Contagious ecthyma (contagious pustular dermatitis; orf) is a common epitheliotrophic viral disease of sheep, goats, and wild ruminants and is characterized by the formation of papules, nodules, or vesicles that progress into thick crusts or heavy scabs on the lips, gingiva, and tongue. A few studies have been conducted, molecular epidemiology based on gene sequences of orf virus has not been performed because the population of sheep and goats is low in Korea. Orf virus infection in dairy goats was identified by clinical diagnosis and PCR. A PCR was performed using previously described primers [4,5]. The B2L and VIR gene sequences of the orf virus, Korean strain were aligned with those of parapoxvirus sequences obtained from GenBank using Bioedit software (Ibis Biosciences, Carlsbad, CA, USA). All clinical samples collected from the orf outbreak were positive using the previously described PCR method. Additional PCR reactions were performed to obtain the full-length B2L and partial VIR genes.
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