Abstract
BackgroundM1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of Phyllolobium chinense Fisch flavonoids (PCFF) was assessed via Zebrafish acute inflammation model in vivo and LPS-induced pro-inflammatory M1 macrophage model in vitro.MethodsThe quality control of P. chinense Fisch flavonoids (PCFF) was analyzed by HPLC. Anti-inflammatory effect of PCFF on the acute injured zebrafish was evaluated by the migration of fluorescence labeled macrophages and neutrophils, and the gene expression of inflammatory factors. In addition, the anti-inflammatory mechanism of PCFF was investigated by the related gene expression and related signaling pathway regulation of pro-inflammatory mediators in LPS-induced pro-inflammatory M1 RAW264.7 macrophage.Results P. chinense Fisch flavonoids (PCFF) markedly suppressed macrophage and neutrophil migration and iNOS gene expression in acute injured zebrafish with tail-cutting. PCFF significantly inhibited NO overproduction and iNOS gene overexpression in LPS-sitimulated pro-inflammatory M1 RAW264.7 macrophages. What’s more, PCFF could evidently decrease p65 protein production, but had no effect on the production of P38, JNK and ERK1/2 proteins.Conclusion P. chinense Fisch flavonoids (PCFF) have a remarkable inhibitory effect on the inflammatory response in acute injured zebrafish and LPS-stimulated M1 RAW264.7 macrophage. The pharmacological mechanism may be related to the regulation of NO overproduction and the inhibition of NF-κB/iNOS signaling pathway.
Highlights
Phyllolobium chinense Fisch is the dry mature seeds of Astragalus complanatus R
It is suggested that the main constituents of P. chinense Fisch flavonoids (PCFF)
The three days post fertilization (3 dpf) Tg (Cora1a: EGFP/Lyz: DsRED) zebrafish were treated with PCFF (15, 30, 50, 100 mg/ml) for 24 h to observe the survival of zebrafish
Summary
Phyllolobium chinense Fisch is the dry mature seeds of Astragalus complanatus R. Br. The main chemical constituents of P. chinense Fisch include flavonoids, triterpenoid saponins, sterols and so on. Flavonoids are the main active ingredients of P. chinense Fisch. It has been showed that P. chinense Fisch flavonoids (PCFF) has many pharmacological effects, such as lowering lipid, bringing high blood pressure down, reducing blood sugar, inhibiting inflammation, improving immunity, etc. The antiinflammatory mechanism of PCFF is still unclear. M1 macrophage plays an important role in inflammatory reaction. Potential anti-inflammatory effect of Phyllolobium chinense Fisch flavonoids (PCFF) was assessed via Zebrafish acute inflammation model in vivo and LPS-induced proinflammatory M1 macrophage model in vitro
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