Abstract
In recent years, the exploitation of nanomaterials in biomedical research has been growing and the demand for development of environmentally benign novel methods for the preparation of such materials is constantly being developed. In the present study, we report the facile synthesis of highly active and stable nanostructured silver (Ag) using the unexplored green marine macroalgae Enteromorpha intestinalis. The nanodimensional properties of the nanostructured Ag were determined using Ultra Violet–visible spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), High Resolution Transmission Electron Microscopy (HRTEM), Selected Area Electron Diffraction (SAED), Energy Dispersive X-ray Spectroscopy (EDX) and Dynamic Light Scattering (DLS) analysis. The formation of nanostructured Ag was confirmed by surface plasmon resonance peak at 436 nm by UV visible spectroscopy. FESEM and EDX analysis showed the purity and polydispersed nature of the particles, while DLS and HRTEM analysis revealed the face-centered, cubic structured particles, spherical in shape with an average particle size of 10–20 nm. The nanostructured Ag was evaluated for their microbicidal behavior in both bacterial and fungal pathogens of clinical importance using standard microbiological guidelines and were found to exhibit potent microbicidal effects on both the system at lesser concentrations.
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