Phycocyanobilin positively modulates inflammatory genes in acute cerebral hypoperfusion in rats

Abstract

Event Abstract Back to Event Phycocyanobilin positively modulates inflammatory genes in acute cerebral hypoperfusion in rats Javier M. Prida1*, Nancy P. Fuentes2, Alexey L. Arzuaga3, Julio R. Massó3, Alieski C. Ramírez3, Nadia Polentarutti4, Federica Riva5, Eduardo P. Arias3, Alberto Mantovani4 and Giselle P. Rol3 1 Center for Research and Biological Evaluations, Institute of Pharmacy and Food, University of Havana, Cuba 2 International Center for Neurological Restoration, Cuba 3 Center for Genetic Engineering and Biotechnology, Cuba 4 Istituto Clinico Humanitas, Italy 5 Department of Veterinary Science and Public Health, University of Milano, Italy Stroke causes high mortality rates and serious disability worldwide. Ischemic penumbra rescue is the main objective of acute interventions, such as thrombolysis and neuroprotection, but they show low translational success. Cumulative evidence demonstrates that inflammatory response is involved in the stroke cascade. Here we evaluated the effects of Phycocyanobilin (PCB), the C-Phycocyanin linked tetrapyrrole, on gene expression in the anterior cortex of hypoperfused brains of rats. The drop in cerebral blood flow during the acute phase of this model is comparable to ischemic penumbra, thus offering a reliable tool for studying molecular mechanisms. The animals were subjected to permanent bilateral common carotid artery occlusion, and then treated with saline or PCB for 30 min, 1, 3 and 6h post-surgery. Samples were taken 24h after the surgery, and processed for RNA extraction. The analysis of global gene expression was carried out with GeneChip Rat Gene ST 1.1 from Affymetrix, and qPCR served to assess the expression of particular genes by using the Fast SYBR Green PCR Master Mix method. The PCB treatment significantly modulated 190 genes (93 up- and 97 down-regulated) associated to several immunological processes in both the regulatory and effector subsets. The qPCR analysis revealed a positive PCB modulation of 13 genes that were mostly related to a detrimental pro-inflammatory environment. Our results support the view of major inflammatory mediators in acute cerebral hypoperfusion, effectively counteracted by PCB. These results may justify the application of PCB as a new acute disease modifying drug against ischemic stroke. Keywords: Phycocyanobilin, Stroke, Inflammatory Response, Neuroprotection, neuroimmunology Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Prida JM, Fuentes NP, Arzuaga AL, Massó JR, Ramírez AC, Polentarutti N, Riva F, Arias EP, Mantovani A and Rol GP (2013). Phycocyanobilin positively modulates inflammatory genes in acute cerebral hypoperfusion in rats. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00391 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Javier M Prida, Center for Research and Biological Evaluations, Institute of Pharmacy and Food, University of Havana, Havana, Cuba, jmarin@infomed.sld.cu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Javier M Prida Nancy P Fuentes Alexey L Arzuaga Julio R Massó Alieski C Ramírez Nadia Polentarutti Federica Riva Eduardo P Arias Alberto Mantovani Giselle P Rol Google Javier M Prida Nancy P Fuentes Alexey L Arzuaga Julio R Massó Alieski C Ramírez Nadia Polentarutti Federica Riva Eduardo P Arias Alberto Mantovani Giselle P Rol Google Scholar Javier M Prida Nancy P Fuentes Alexey L Arzuaga Julio R Massó Alieski C Ramírez Nadia Polentarutti Federica Riva Eduardo P Arias Alberto Mantovani Giselle P Rol PubMed Javier M Prida Nancy P Fuentes Alexey L Arzuaga Julio R Massó Alieski C Ramírez Nadia Polentarutti Federica Riva Eduardo P Arias Alberto Mantovani Giselle P Rol Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.