Phycocyanin Extraction From Frozen and Freeze-Dried Biomass of Pseudanabaena sp. by Using Mild Cell Disruption Methods

Berke Kisaoğlan,Zeliha Demi̇rel,Meltem Conk Dalay

doi:10.33714/masteb.951265

Abstract

Phycocyanin is a precious, natural, blue coloured pigment-protein complex that has commercial value and wide application in cosmetics, food, and pharmaceutical industries. In the present study, we performed various cell disruption methods (ultrasonication, homogenization, freeze/thaw and CaCl2 extraction) for phycocyanin extraction from different forms of biomass of a thermophilic Pseudanabaena sp. that has a high potential to produce high-quality phycocyanin. Using potassium phosphate buffer and ultrasonic bath method, we achieved the highest phycocyanin yield (345 mgPC.g-biomass) from freeze-dried biomass and we obtained increased yield as the duration of application increases. Phycocyanin yields were calculated as 345 mgPC.g-biomass, 255 mgPC.g-biomass and 220 mgPC.g-biomass for 5, 10 and 15 min, respectively. In this study, cell disruption methods have determined significantly more effective on freeze-dried biomass rather than frozen biomass. Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells.

Highlights

Pseudanabaena species are non-heterocystous cyanobacteria which are characterized by trichomes and cells that are smaller than 2-4 μm in size (Tamburaci, 2009)
Phycocyanin extraction from frozen biomass was performed by implementing different mild cell disruption methods including ultrasonication, homogenization, and freeze/thaw, and CaCl2 extraction (Table 1)
Calculated PC concentration and yields for frozen biomass were critically lower than freeze-dried biomass (Table1 and Table 2). These findings show that extraction yield and 336

Summary

Introduction

Pseudanabaena species are non-heterocystous cyanobacteria which are characterized by trichomes and cells that are smaller than 2-4 μm in size (Tamburaci, 2009). Has been widely investigated in the aspect of phycoerythrin, and in some studies, the phycocyanin production capacity of the species was reported (Tamburaci, 2009; Khan et al, 2019). Having distinct spectral characteristics and bioactive properties (anti-inflammatory, anti-viral, anti-oxidant, etc.) are the main features that make phycobiliproteins widely-applicable natural substances in cosmetics, food, and pharmaceutical industries (Puzorjov & McCormick, 2020). Phycocyanin (PC), Phycoerythrin (PE), and Allophycocyanin (APC) are the subtypes of phycobiliproteins that have distinct spectral characters and commercial value depending on their purity and stability

Methods

Results

Discussion

Conclusion