PHYCOBILIPROTEIN COMPONENTS AND CHARACTERISTICS OF THE PHYCOBILISOME FROM A THERMOPHILIC CYANOBACTERIUM MYXOSARCINA CONCINNA(1).

Abstract

A phycocyanin (PC) and three allophycocyanin (AP) components (designated PC, AP1, AP2, and AP3) were prepared from Myxosarcina concinna Printz phycobilisomes by the native gradient PAGE performed in a neutral buffer system combined with the ion exchange column chromatography on DEAE-DE52 cellulose. PC contained one β subunit () and two α ones ( and ), and it carried two rod linkers ( and ) and one rod-core linker (). AP1 and AP3 were characterized as peripheral core APs, whereas AP2 was an inner-core one. AP2 and AP3 were demonstrated to function as the terminal emitters. Each of the three APs contained two β subunits ( and ), two α subunits ( and ) and an inner-core linker (). AP2 and AP3 had another subunit of the allophycocyanin B (AP-B) type () belonging to the β subunit group, and AP1 and AP3 carried their individual specific core linkers ( and ), respectively. No AP component was shown to associate with the core-membrane linker LCM . The functions of the linker polypeptides in the phycobilisome (PBS) construction are discussed.