Phrenic responses to isocapnic hypoxia in adult rats following perinatal hyperoxia

Abstract

The purpose of this study was to test the hypothesis that carotid body-mediated, phrenic nerve responses to hypoxia are attenuated in adult rats that had been previously exposed to perinatal hyperoxia (one month of 60% O 2; perinatal treated rats). Integrated phrenic nerve responses to strictly controlled isocapnic hypoxia were measured in urethane-anesthetized, vagotomized, paralyzed and ventilated adult rats 2–5 months after perinatal hyperoxia, before and after bilateral carotid denervation. In untreated control rats, phrenic burst frequency, peak amplitude of integrated phrenic activity and minute phrenic activity increased 21±3 bursts/min (mean±SE), 158±20% and 279±34%, respectively, during hypoxia (50 Torr Pa O 2 ). In contrast, phrenic nerve activity increased to a significantly lesser degree in perinatal treated rats (frequency, 12±2 bursts/min; amplitude, 87±13%; minute activity, 150±19%; all P<0.05). Hypoxic phrenic responses were abolished by carotid denervation in both rat groups. In rats exposed to hyperoxia as adults, hypoxic phrenic responses were not attenuated versus untreated control rats. The data indicate that carotid body-mediated, isocapnic hypoxic chemoreflexes are impaired in perinatal treated rats, an effect unique to development. These effects cannot be accounted for by differences in blood gases (O 2 or CO 2) or pulmonary mechanics.