PHOTOTRANSFORMATION KINETICS OF UNDEGRADED OAT AND PEA PHYTOCHROME INITIATED BY LASER FLASH EXCITATION OF THE RED‐ABSORBING FORM

Abstract

Abstract— Phototransformation at 2°C of the red‐absorbing form of phytochrome (Pr) to the far‐redabsorbing form (Pfr) was studied with both undegraded oat (Avena sativa L., cv. Garry) and undergraded pea Pisum sativum L., cv. Alaska) phytochrome. Phototransformation was initiated by a 15‐ns laser pulse with maximum emission near 600 nm and output power of 30 mJ. The first resolvable transformation intermediate exhibited relative to Pr a maximum absorbance increase near 700 nm and was fully present at the earliest time measured, which was 60 ns after the flash. This intermediate absorbance decayed by two reactions for oat phytochrome (half‐lives of 11 and 140 μs assuming parallel reactions) and by three for pea phytochrome (half‐lives of 14, 280 and 1600 μs assuming parallel reactions). The kinetics of the slowest reaction for pea phytochrome, however, might be somewhat distorted by an instrument artifact. The appearance of the far‐red‐absorbing phytochrome, as monitored by absorbance increase at 720 nm, occurred by at least two reactions for both oat (half‐lives of 47 and 250 ms assuming parallel reactions) and pea (half‐lives of 170 and 770 ms assuming parallel reactions) phytochrome. The possibility of slower reactions was not tested. Assays for possible proteolysis of the phytochrome samples studied here indicated that the presence of degraded phytochrome could not account for the observed multiphasic kinetics except possibly for one phase of the triphasic intermediate decay seen with pea phytochrome.