Abstract
We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest laser pulse energy and convert it into a localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. Integrating the metallic nanostructure with a micropipette, the nanoblade generates a micron-sized membrane access port for delivering concentrated cargo (5×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">8</sup> bacteria/ml) with high efficiency (46%) and cell viability (>;90%) into mammalian cells. Additional biologic and inanimate cargo over 3-orders of magnitude in size including DNA, 200 nm polystyrene beads to 2 μm bacteria have been delivered into multiple cell types.
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