Abstract
The potential of photothermal beam deflection spectrometry (PTDS), photoacoustic spectroscopy (PAS) and photothermal radiometry (PTR) for the characterization of thin-layer chromatography (TLC) plates with respect to the surface and in-depth distribution of different compounds inside the sorbent was investigated. Photothermal measurements of TLC chromatograms of the Camag III test dye mixture demonstrate that accurate and comparable values for thermal diffusivity of spots in chromatograms and of the sorbent can be obtained by PTDS and PAS. PAS has been demonstrated to be the most reliable and least time-consuming among the three investigated techniques. Differences observed between the PAS signal and the phase-frequency scans obtained from the spots of the same compound on different TLC plates could be due to the irreproducibility of chromatogram development or the inhomogeneousness in different TLC plates.
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