Photosynthetic recovery in the resurrection plant Selaginella lepidophylla after wetting.

Abstract

Photosynthetic recovery (PR) in a southwest Texas, USA population of Selaginella lepidophylla (Hook and Grev.) (Selaginellaceae), a poikilohydric spikemoss, was examined in the laboratory. Infrared CO2 gas analysis and ribulose 1,5-bisphosphate (RuBP) carboxylase activity measurements indicated that optimal temperature for PR was near 25°C in terms of: (1) rapidity of net CO2 uptake after hydration (5.4 h), (2) maximum net photosynthetic rate at 2000 μE·m-2·s-1 (2.44 mg CO2·g(DWT)-1·h-1), and (3) maximum net CO2 assimilation per 30 h hydration event (43.8 mg CO2·g(DWT)-1·30 h-1). The PR was much slower at both 15° and 35° C, with lower photosynthetic rates and net carbon gains per hydration event. High respiratory costs were incurred at 45°C and no net photosynthesis was observed. Increases in RuBP carboxylase activity and chlorophyll content during 24 h hydration were also greatest near 25°C. Dry plants had 60% of the enzyme activity of fully recovered (24 h hydration) plants, indicating enzyme conservation. Actinomycin D and cycloheximide did not appear to inhibit PR, but chloramphenicol appeared to totally inhibit RuBP carboxylase activity increases over levels conserved in dry plants. Therefore, rapid PR in S. lepidophylla was achieved by both rapid increase in RuBP carboxylase activity, possibly via de novo synthesis, and conservation of the photosynthetic enzyme. Both mechanisms are essential to maximize assimilation in S. lepidophylla in an environment where hydrated periods are rare and of short duration.