Photosynthetic plasticity allows blueberry (Vaccinium corymbosum L.) plants to compensate for yield loss under conditions of high sink demand

Abstract

In some growing environments blueberry (Vaccimium corymbosum L.) yields exhibit excessive annual variation associated with poor photosynthetic performance. The purpose of this study was to determine how photosynthesis may be affected by demand and to define the mechanisms underpinning photosynthetic plasticity. Manipulation of source-sink ratios revealed that yields were maintained following 50 % defoliation. This was associated with an adaptive increase in photosynthetic capacity mediated via changes in stomatal physiology, photosynthetic electron transport and CO2 assimilation. Transcripts encoding enzymes of the Calvin-Benson cycle including the Rubisco large subunit, Rubisco activase, phosphoribulokinase and plastid-localised glyceraldehyde phosphate dehydrogenase 3 were more abundant in leaves from partially defoliated plants relative to control plants. Short-term 13CO2 labelling experiments suggested that partial defoliation did not incur an assimilation penalty although the accumulation of sugars and starch in some organs was reduced. Metabolite profiles of leaves from partially defoliated plants exhibited some differences from those of control leaves, however, no changes in the diurnal content of leaf sugar and starch were observed between treatments. The data highlights the mechanisms by which blueberry leaves adapt to increased demand and demonstrate that photosynthetic plasticity can compensate for significant loss of the photosynthetic area.