Photosynthetic inner antenna CP47 plays important roles in ephemeral plants in adapting to high light stress

Abstract

Throughout 3.5 billion years of evolution, photosynthesis of land plants has developed a complicated antenna system to cope with the ever-changing environments. The antenna system of photosystem (PS) II includes the outer antennae and inner antennae. The inner antennae CP43 and CP47, located in the closest peripheral of PSII reaction center (RC), play important roles in facilitating excitation energy transport from the outer antennae to the PSII RC. Although PSII RC is the last station of energy transport, it is the inner antenna CP47, not the RC, which possesses the lowest energy level in PSII. Berteroa incana (B. incana), which is a vascular plant grown in the Gobi region, can sustain very high photosynthesis capacity under very high light conditions. It has been discovered that the thylakoid membrane of B. incana possesses a unique low fluorescence emission spectrum because the fluorescence emission of CP47 (695 nm) is the main fluorescence emission peak of PSII. In this paper, the thylakoid membrane, isolated from B. incana grown under a light condition of 100 μM photons m−2 s-1 and subjected to high light treatment (1600 μM photons m-2 s-1 for 1.5 h or 3 h) was employed for the research. It has been found that the high fluorescence emission of CP47 decreased remarkably upon exposure to the high light treatment. Further observation revealed that the drastic changes in the CP47 fluorescence emission were accompanied by a slight reduction in the amount of CP47 and an enhancement of the CP29-LHCII-CP24 assembly. It is proposed that CP47 enables the functional switch between the excitation energy transfer towards PSII RC, and the overexcitation quenching in the PSII core. Together with CP43, CP47 plays important roles in regulating excitation energy distribution in PSII core complexes.