Photosynthetic characteristics and protective mechanisms against photooxidation during high temperature stress in two citrus species

Abstract

Exposure of Satsuma mandarin ( Citrus unshiu Marc.) and Navel orange ( Citrus sinensis Osbeck) plants to high temperature (38 °C) led to reductions of the net photosynthetic rate (Pn), the photorespiration rate (Pr), the quantum efficiency CO 2 assimilation ( Φ C O 2 ), the maximal photochemical efficiency of PS2 (Fv/Fm), the photochemical quenching (qP) and the quantum efficiency of PS2 photochemistry ( Φ PS2), whereas the minimal fluorescence yield (Fo) and the non-photochemical quenching (qN) increased. Increase in the value of Pr/Pn and Φ PS 2 / Φ C O 2 was attributed to the greater decrease in Pn and Φ C O 2 than Pr and Φ PS2. In addition, the superoxide radical (O 2 −) production, the H 2O 2 concentration and the activities of antioxidant enzymes such as the superoxide dismutase (SOD, EC 1.15.1.1), the ascorbate peroxidase (APX, EC 1.11.1.11), the dehydroascorbate reductase (DHAR, EC 1.8.5.1) and the catalase (CAT, EC 1.11.1.6) were raised. On the other hand, the chlorophyll concentration in leaves decreased during high temperature stress. These results suggest that decline in Pn related to inactivation of PS2 reaction centers may be due to the enhanced number of active oxygen species in the citrus leaves. The water–water cycle may play a role in limiting the degree of photodamage caused by high temperature. Lower O 2 − production rate, the H 2O 2 concentration and the antioxidant enzymes activity were observed in high temperature tolerant species of citrus. The exogenous active oxygen scavenger ascorbic acid (Asc) enhanced the ability to clear the O 2 − in citrus plants, and quicken the recovery of photosynthetic apparatus.