Abstract
Proliferating cultures of Actinidia deliciosa cv Tomuri were grown in vitro under a photosynthetic photon flux density (PPFD) of 120 μmol m 2/s. Some jars were daily enriched with 2000 μl/l CO 2 administered at the end of the dark period. Head space analysis revealed that CO 2 accumulated up to 9500 μl/l during the dark period and was drastically reduced by photosynthetic activity to 150–200 μl/l during the photoperiod without any significant difference between CO 2-enriched and non-enriched cultures. Oxygen concentration assayed at the end of the photoperiod showed a steady increase during the 44 days of culture and was not reduced to atmospheric values by respiratory processes during the dark period. CO 2 enrichment enhanced O 2 production and accumulation to 32.5% at the end of the culture period. Oxygen photoreduction and its photo-oxidative damage to green tissue cells are discussed.
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