Photosynthetic bacteria with iron oxide nanoparticles as catalyst for cooking oil removal and valuable products recovery with heavy metal co-contamination

Abstract

Waste cooking oil discharge causes environmental pollution in receiving waters, particularly when associated with heavy metals that can lead to formation of hazardous organometallic compounds. This study combined iron oxide nanomaterial and the anoxygenic photosynthetic bacterium Rhodopseudomonas faecalis PA2 for removal of cooking oil in the presence of heavy metals. R. faecalis PA2, with known capability to generate beneficial substances from several wastes, was capable of cooking oil removal with production of valuable products. Oil removal, biomass, protein, and carotenoid production were 82.38%, 1.48 g/L, 1,600.19 mg/L, and 1,046.33 mg/L, respectively, under optimal conditions (cooking oil as carbon source and 30% inoculum density). Iron (Fe) stimulates growth of R. faecalis; in this study, Fe3O4 nanoparticles were synthesized and used as a catalyst to facilitate interaction and high reactivity between Fe and R. faecalis PA2. Size measurement by transmission electron microscopy (17.44 nm), X-ray diffraction peaks, and magnetic susceptibility confirmed that the synthesized nanoparticles were magnetite Fe3O4. Biomass, protein, and carotenoid production of the Fe3O4 supplemented experiment increased by 61.56%, 70.78%, and 57.2%, respectively, when compared with the control. When different concentrations of heavy metals (Pb, Ni, Co, and Zn) were supplemented in the media containing cooking oil, Fe3O4 addition increased heavy metal tolerance, improved bacterial growth, and enhanced valuable products when compared with the non-supplemented group. This study reports the positive impact of nanoparticle application as a catalyst for valorization of cooking oil waste with heavy metal co-contamination by the photosynthetic bacterium R. faecalis PA2.