Photosynthetic and Morphological Acclimation to High and Low Light Environments in Petasites japonicus subsp. giganteus

Ray Deguchi,Kohei Koyama

doi:10.3390/f11121365

Abstract

Within each species, leaf traits such as light-saturated photosynthetic rate or dark respiration rate acclimate to local light environment. Comparing only static physiological traits, however, may not be sufficient to evaluate the effects of such acclimation in the shade because the light environment changes diurnally. We investigated leaf photosynthetic and morphological acclimation for a perennial herb, butterbur (Petasites japonicus (Siebold et Zucc.) Maxim. subsp. giganteus (G.Nicholson) Kitam.) (Asteraceae), in both a well-lit clearing and a shaded understory of a temperate forest. Diurnal changes in light intensity incident on the leaves were also measured on a sunny day and an overcast day. Leaves in the clearing were more folded and upright, whereas leaves in the understory were flatter. Leaf mass per area (LMA) was approximately twofold higher in the clearing than in the understory, while light-saturated photosynthetic rate and dark respiration rate per unit mass of leaf were similar between the sites. Consequently, both light-saturated photosynthetic rate and dark respiration rate per unit area of leaf were approximately twofold higher in the clearing than in the understory, consistent with previous studies on different species. Using this experimental dataset, we performed a simulation in which sun and shade leaves were hypothetically exchanged to investigate whether such plasticity increased carbon gain at each local environment. As expected, in the clearing, the locally acclimated sun leaves gained more carbon than the hypothetically transferred shade leaves. By contrast, in the understory, the daily net carbon gain was similar between the simulated sun and shade leaves on the sunny day due to the frequent sunflecks. Lower LMA and lower photosynthetic capacity in the understory reduced leaf construction cost per area rather than maximizing net daily carbon gain. These results indicate that information on static photosynthetic parameters may not be sufficient to evaluate shade acclimation in forest understories.

Highlights

In forests, individual plants from a single species often experience various light environments, from well-lit clearings or large gaps to shaded understories [1,2,3,4]
Plants grown in shaded places have leaves with a lower light-saturated photosynthetic rate [1,2,3,4,5,6,7,15,18,19,20,26,27,28,29,30,31] and a lower dark respiration rate [1,2,3,4,5,6,7,18], have thinner leaves with a lower leaf mass per unit area associated with their lower biomass investment per unit area [4,15,18,19,20,27,28,29,32,33,34], and have a higher leaf mass ratio [5,18,25,28] than plants grown in well-lit places
The differences in the light environment were quantified by large differences in relative PPFD (rPPFD) and daily light integral between the two sites (Table 1)

Summary

Introduction

In forests, individual plants from a single species often experience various light environments, from well-lit clearings or large gaps to shaded understories [1,2,3,4]. As sessile organisms, phenotypic plasticity is essential for survival in such heterogeneous environments [3,5,6,7,8] This phenotypic plasticity and the consequent intraspecific variation greatly influence community-level plant traits and productivity [9,10,11,12,13,14,15], highlighting the importance of the quantification of phenotypic plasticity of plant traits under different light environments. Plants grown in shaded places have leaves with a lower light-saturated photosynthetic rate [1,2,3,4,5,6,7,15,18,19,20,26,27,28,29,30,31] and a lower dark respiration rate [1,2,3,4,5,6,7,18], have thinner leaves with a lower leaf mass per unit area associated with their lower biomass investment per unit area [4,15,18,19,20,27,28,29,32,33,34], and have a higher leaf mass ratio (i.e., leaf mass relative to whole-plant mass) [5,18,25,28] than plants grown in well-lit places

Methods

Results

Discussion

Conclusion