Abstract

The influence of the stress factors pH and temperature on photosynthesis and photohydrogen evolution was investigated in a unicellular marine green alga, Chlorococcum littorale. Maximum activities of photosynthetic oxygen evolution and photohydrogen evolution were determined at 5% CO 2, pH 7.5 and 25 °C. Increases in pH or temperature caused damage of photosystem II, whereas the photohydrogen-producing system was much less affected. At pH 11, a decrease in photosynthetic oxygen evolution was observed, caused by partial inhibition of the water-splitting complex. This led to photohydrogen evolution lasting for more than 1 h. At high temperatures, both photosynthesis and H 2 evolution became unstable.

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