Abstract
DURING work with iron chelates, it was noted that many chemical analyses for total iron were inconsistent when repeated at intervals on solutions left standing on the laboratory bench in the light. A sensitive method was then devised for the estimation of the chelated iron molecule as a whole, based on the light-absorption of solutions in the ultra-violet region. Dilute solutions (approximately 0.0001 M) of the di-sodium iron ethylenediaminetetracetate have an absorption curve with a maximum at about 260 mµ. This peak is well separated from, and is much more pronounced than, that of the di-sodium ethylenediaminetetracetate itself at about 210 mµ. Therefore, the optical density at 260 mµ. as determined by a spectrophotometer, was used as a measure of the chelated iron present in solutions of the di-sodium iron compound.
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