Abstract
Polycaboxymethylbetaine (PCMB) having a carboxymethylbetaine (CMB) and epoxy structure on its side chains was synthesized. The photosensitive anti-biofouling coating was formulated by loading the PCMB with a photo acid generator (PAG). By applying i-line irradiation and subsequent baking to this coating film, the processed area of the former film became insoluble to water, and thus, photolithographic polymer micropatterns with various dimensions were created on the substrates. As an initial assessment for targeted three-dimensional (3D) culture, HT-29 human colon cancer cells and UE7T-13 human mesenchymal stem cells (hMSCs) were disseminated on the patterns, and then cultured. Results showed that both cells selectively aggregated within the patterns. The cell aggregates prepared here may be promising candidates for use in regenerative medicine and/or drag screening.
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