Abstract
A novel concept of photoresponsive ion-selective optical sensor is described. Photochemical reactions can be utilized to generate and control ion fluxes in an ion-selective optode in the same manner as nonequilibrium electrochemical methods have been used in ion-selective electrodes. In contrast to their equilibrium counterparts, the photoresponsive pH-selective ion optodes are sensitive to both the buffer capacity of the sample and activity of hydrogen ions. Active optical probes are especially attractive for intracellular applications because they can be fabricated as submicron-sized beads. Common optical techniques, such as fluorescence microscopy and flow cytometry, can be combined with active ion probes with only minor modification of the existing experimental setup.
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