Photorespiration is the major alternative electron sink under high light in alpine evergreen sclerophyllous Rhododendron species

Abstract

Owing to the high leaf mass per area, alpine evergreen sclerophyllous Rhododendron have low values of mesophyll conductance (gm). The resulting low chloroplast CO2 concentration aggravates photorespiration, which requires a higher ATP/NADPH ratio. However, the significance of photorespiration and underlying mechanisms of energy balance in these species are little known. In this study, eight alpine evergreen sclerophyllous Rhododendron species grown in a common garden were tested for their gm, electron flow to photorespiration, and energy balancing. Under saturating light, gm was the most limiting factor for net photosynthesis (AN) in all species, and the species differences in AN were primarily driven by gm rather than stomatal conductance. The total electron flow through photosystem II (ETRII) nearly equaled the electron transport required for Rubisco carboxylation and oxygenation. Furthermore, blocking electron flow to photosystem I with appropriate inhibitors showed that electron flow to plastic terminal oxidase was not observed. As a result, these studied species showed little alternative electron flow mediated by water-water cycle or plastic terminal oxidase. By comparison, the ratio of electron transport consumed by photorespiration to ETRII (JPR/ETRII), ranging from 43%∼55%, was negatively correlated to AN and gm. Furthermore, the increased ATP production required by enhanced photorespiration was regulated by cyclic electron flow around photosystem I. These results indicate that photorespiration is the major electron sink for dissipation of excess excitation energy in the alpine evergreen sclerophyllous Rhododendron species. The coordination of gm, photorespiration and cyclic electron flow is important for sustaining leaf photosynthesis.