Photorespiration in C3?C4 intermediate species of Alternanthera and Parthenium: Reduced ammonia production and increased capacity of CO2 refixation in the light

Abstract

The pattern of photorespiratory ammonia (PR-NH3) formation and its modulation by exogenous bicarbonate or glycine were investigated in C3-C4 intermediates of Alternanthera (A. ficoides and A. tenella) and Parthenium hysterophorus in comparison to those of C3 or C4 species. The average rates of PR-NH3 accumulation in leaves of the intermediates were slightly less than (about 25% reduced) those in C3 species, and were further low in C4 plants (40% of that in C3). The levels of PR-NH3 in leaf discs decreased markedly when exogenous bicarbonate was present in the incubation medium. The inhibitory effect of bicarbonate on PR-NH3 accumulation was pronounced in C3 plants, very low in C4 species and was moderate in the C3-C4 intermediates. Glycine, an intermediate of photorespiratory metabolism, raised the levels of PR-NH3 in leaves of not only C4 but also C3-C4 intermediates, bringing the rates close to those of C3 species. The rate of mitochondrial glycine decarboxylation in darkness in C3-C4 intermediates was partially reduced (about 80% of that in C3 species), corresponding to the activity-levels of glycine decarboxylase and serine hydroxymethyltransferase in leaves. The intermediates had a remarkable capacity of reassimilating photorespiratory CO2 in vivo, as indicated by the apparent refixation of about 85% of the CO2 released from exogenous glycine in the light. We suggest that the reduced photorespiration in the C3-C4 intermediate species of Alternanthera and Parthenium is due to both a limitation in the extent of glycine production/decarboxylation and an efficient refixation/recycling of internal CO2.