Abstract

1. 1. No photoreactivation (PR) was observed in dry cysts of Colpoda inflata irradiated with either monochromatic 265 nm far UV radiation or with polychromatic radiation from an argon plasma arc lamp filtered through a sapphire window with a cutoff at 145 nm. 2. 2. Wet Colpoda cysts, however, showed a high degree of PR, especially after large UV doses from either source listed above. PR was achieved using two lamps, each with two 4 watt cool white fluorescent bulbs 8 cm from the cyst preparation; an exposure of 4 or more hours was required for maximal PR. 3. 3. Wet cysts were damaged by much smaller doses of far UV than were dry cysts; such damage is, however, also subject to PR. 4. 4. It is probable that PR fails in dry cysts because the photoenzyme which provides energy for PR by absorbing visible and near UV radiation is unable to act in the absence of water.

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